Microbiological Profile of Bloodstream Infections in ICU Sepsis Patients: A Hospital-Based Study

Muayad Mahdi Kadim; Ali Abedulameer Alhusayni; Falah Hasan Obayes AL-Khikani

المؤلفون

Muayad Mahdi Kadim Department of Microbiology, Al-shomali general hospital, Babylon Health directorate, Babylon, Iraq.
Ali Abedulameer Alhusayni Department of Microbiology, Al-shomali general hospital, Babylon Health directorate, Babylon, Iraq.
Falah Hasan Obayes AL-Khikani 1. Department of Microbiology, Al-Shomali General Hospital, Babylon Health Directorate, Babylon, Iraq 2. Department of Medical Microbiology, College of Dentistry, University of Cordoba, Najaf, Iraq. 2. Department of Medical Laboratory Technology, College of Medical Technology, Islamic University, Najaf, Iraq.

الكلمات المفتاحية:

Pseudomonas stutzeri; bloodstream infection; community-onset; epidemiology; mortality, Bacteremia, BSIs, sepsis.

الملخص

Background: Severe sepsis remains a critical medical condition with high morbidity and mortality rates. Identifying the causative pathogens is essential for effective treatment. This study aimed to determine the bacterial profile isolated from blood cultures of severe sepsis patients admitted to intensive and respiratory care units in Al-Hilla hospitals.

Methods: A total of 100 severe sepsis patients (aged 13–90 years) were enrolled from August 2023 to January 2024. Blood samples (7 mL) were collected from patients with abnormal complete blood counts (CBC) and C-reactive protein (CRP) levels >10 mg/L. Blood cultures were cultured on selective media (blood agar, MacConkey, etc.), then processed using the automated VITEK2 system (bioMérieux), followed by Ethical approval was obtained from the Babylon Health Directorate.

Results: Among 100 patients (mean age 59.60 ± 18.314 years), 25 (25%) had positive blood cultures. The most prevalent isolates were Stenotrophomonas maltophilia and Pseudomonas stutzeri (16% each), followed by Kocuria kristinae (12%). Staphylococcus aureus, S. epidermidis, and Escherichia coli each accounted for 8%, while other species (Streptococcus pneumoniae, Salmonella typhi, etc.) were detected in single cases (4% each).

Conclusion: Gram-negative bacteria, particularly S. maltophilia and P. stutzeri, were the predominant pathogens in sepsis patients. Continuous surveillance of microbial patterns is crucial for guiding empirical antibiotic therapy and improving clinical outcomes in sepsis management.